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Background: Diabetic foot infection (DFI) is an infection in soft tissue or bone in patient with diabetes mellitus, Citrobacter species are infrequent nosocomial pathogens and cause a range of infections. the aim of the current study we report the isolation of carbapenem genes among Citrobacter species isolated from DFI. Methods: about 111 swabs specimen were collection from patient suspected with DFI during the period of study, determine the antibiotic susceptibility of Citrobacter spp isolates by using Kirby-Bauer disk diffusion method to 23 antimicrobial agents according to the CLSI (2021). Molecular detection for MBL genes by multiplex PCR. Results: from 111 swabs specimen Citrobacter spp isolates had represented in 3 (4.34%) among Gram-negative bacteria. Antibiotic susceptibility test revealed that one isolate of Citrobacter braakii was MDR and the other isolates was PDR. The PCR data of MBL genes revealed that the frequency of MBL genes among Citrobacter braakii as following blaVIM, blaNDM, and blaSIM were 100%. Conclusion: the study show the appear MDR and PDR among Citrobacter species and Citrobacter braakii isolate carry the MBL genes (blaVIM, blaNDM,, and blaSIM) where the dissemination of NDM producing Citrobacter braakii considered first reported in Iraq.
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- • Ali, M. S. (2020). Antibiotic Resistant Patterns of Bacteria Recovered from Infected Diabetic Foot as Correlated to Risk Factors. M.sc.Thesis.Faculty of pharmacy.Isra University.
- • Alshara, J. M. R., Alsehlawi, Z. S. R., & Aljameel, D. S. A. (2014). First Report of New Delhi Metallo-beta-Lactamase (NDM-1) Producing Pseudomonas aeruginosa in Iraq. Journal of Biology, Agriculture and Healthcare, 4(14), 40–47. http://www.iiste.org/Journals/index.php/JBAH/article/view/13975
- • Armstrong, D. G., Swerdlow, M. A., Armstrong, A. A., Conte, M. S., Padula, W. V, & Bus, S. A. (2020). Five year mortality and direct costs of care for people with diabetic foot complications are comparable to cancer. Journal of Foot and Ankle Research, 13(1), 1–4.
- • Barberino, M. G., Cruvinel, S. de A., Faria, C., Salvino, M. A., & Silva, M. de O. (2018). Isolation of blaNDM-producing Enterobacteriaceae in a public hospital in Salvador, Bahia, Brazil. Brazilian Journal of Infectious Diseases, 22(1), 47–50. https://doi.org/10.1016/j.bjid.2017.10.002
- • Braun, S., & Silva, M. (2021). Clinical and microbiological characteristics of diabetic foot infection by carbapenem-resistant bacteria in a hospital in southern Brazil. Evidência, 1–10. https://doi.org/10.18593/eba.27178
- • Duceppe, M., Phipps-Todd, B., Carrillo, C., & Huang, H. (2019). Draft Genome Sequences of Eight Canadian Citrobacter braakii and Citrobacter freundii Strains. Microbiology Resource Announcements, 8(27), 11–13. https://doi.org/10.1128/mra.00273-19
- • Goh, T. C., Bajuri, M. Y., Nadarajah, S. C., Rashid, A. H. A., Baharuddin, S., & Zamri, K. S. (2020). Clinical and bacteriological profile of diabetic foot infections in a tertiary care. Journal of Foot and Ankle Research, 13(1), 1–8.
- • Han, H., Zhao, Z., Lin, Y., Lin, B., Xu, H., & Zheng, B. (2022). Co-Production of NDM-1 and OXA-10 β-Lactamase in Citrobacter braakii Strain Causing Urinary Tract Infection. Infection and Drug Resistance, 15(March), 127–133. https://doi.org/10.2147/IDR.S347943
- • Hart, C. A. (2006). Klebsiella, citrobacter, enterobacter and serratia spp. Principles and Practice of Clinical Bacteriology, 377–386.
- • Kow, R. Y., Low, C. L., Ayeop, M. A. S., Che-Ahmad, A., & Awang, M. S. (2022). Characteristics and Microbiological Profile of Patients with Diabetic Foot Infections in Kuantan, Pahang. Malaysian Orthopaedic Journal, 16(1), 11–17. https://doi.org/10.5704/MOJ.2203.003
- • Kumar Trivedi, M., & Branton, A. (2015). Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen. Journal of Clinical & Medical Genomics, 3(1), 0–8. https://doi.org/10.4172/2472-128x.1000129
- • Leylabadlo, H. E., Asgharzadeh, M., & Aghazadeh, M. (2015). Dissemination of carbapenemases producing Gram negative bacteria in the Middle East. Iranian Journal of Microbiology, 7(5), 226.
- • Li, X., Du, Z., Tang, Z., Wen, Q., Cheng, Q., & Cui, Y. (2022). Distribution and drug sensitivity of pathogenic bacteria in diabetic foot ulcer patients with necrotizing fasciitis at a diabetic foot center in China. BMC Infectious Diseases, 22(1), 1–10. https://doi.org/10.1186/s12879-022-07382-7
- • MacFaddin, J. F. (2000). Biochemical Tests for Identification of Bacteria of Medical Importance. Lippincott Williams & Wilkins. 3rd. edition. USA.
- • Pepperell, C., Kus, J. V., Gardam, M. A., Humar, A., & Burrows, L. L. (2002). Low-virulence Citrobacter species encode resistance to multiple antimicrobials. Antimicrobial Agents and Chemotherapy, 46(11), 3555–3560. https://doi.org/10.1128/AAC.46.11.3555-3560.2002
- • Poirel, L., Fortineau, N., & Nordmann, P. (2011). International transfer of NDM-1-producing Klebsiella pneumoniae from Iraq to France. Antimicrobial Agents and Chemotherapy, 55(4), 1821–1822.
- • Poirel, L., Walsh, T. R., Cuvillier, V., & Nordmann, P. (2011). Multiplex PCR for detection of acquired carbapenemase genes. Diagnostic Microbiology and Infectious Disease, 70(1), 119–123.
- • Sami, H., Sultan, A., Rizvi, M., Khan, F., Ahmad, S., Shukla, I., & Khan, H. (2017). Citrobacter as a uropathogen, its prevalence and antibiotics susceptibility pattern. CHRISMED Journal of Health and Research, 4(1), 23. https://doi.org/10.4103/2348-3334.196037
- • Sen, P., & Demirdal, T. (2020). Evaluation of mortality risk factors in diabetic foot infections. International Wound Journal, 17(4), 880–889. https://doi.org/10.1111/iwj.13343
- • Shabhay, A., Horumpende, P., Shabhay, Z., Mganga, A., Van Baal, J., Msuya, D., Chilonga, K., & Chugulu, S. (2021). Clinical profiles of diabetic foot ulcer patients undergoing major limb amputation at a tertiary care center in North-eastern Tanzania. BMC Surgery, 21(1), 1–7.
- • Walsh, T. R. (2005). The emergence and implications of metallo‐β‐lactamases in Gram‐negative bacteria. Clinical Microbiology and Infection, 11, 2–9.
- • Yao, Y., Falgenhauer, L., Falgenhauer, J., Hauri, A. M., Heinmüller, P., Domann, E., Chakraborty, T., & Imirzalioglu, C. (2021). Carbapenem-Resistant Citrobacter spp. as an Emerging Concern in the Hospital-Setting: Results From a Genome-Based Regional Surveillance Study. Frontiers in Cellular and Infection Microbiology, 11(November), 1–12. https://doi.org/10.3389/fcimb.2021.744431
- • Yong, D., Toleman, M. A., Giske, C. G., Cho, H. S., Sundman, K., Lee, K., & Walsh, T. R. (2009). Characterization of a new metallo-β-lactamase gene, bla NDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrobial Agents and Chemotherapy, 53(12), 5046–5054.