Effect of Iron Intake on Chronic Cholestatic Osteoporosis by Surgical CBD (Common Bile Duct) Partial Ligation

Abstract

Osteoporosis is an important type of metabolic bone disease in chronic cholangiogenic liver disease, with an incidence of up to maximum 72%. Osteoporosis and osteomalacia in patients with chronic liver disease are collectively referred to as "hepatic osteodystrophy," which is commonly found in patients with intestinal malabsorption and progressive primary biliary cirrhosis. To deal with the induction of osteoporosis by chronic cholestasis, we aimed to investigate the following: The aim of the study was to investigate the effect of iron intake on chronic cholestatic osteoporosis by surgical CBD partial ligation. Forty rats including weanling male rats weighing 70-80g and adult male rats weighing 150-160g were used. First, the rats were anesthetized with intraperitoneal ketamine injection, and then the CBD (common bile duct) was exposed by surgical procedures, complete and partial ligation was performed, and then the abdomen was closed. Partial ligation was performed by ligating different thicknesses of the suture needles with the common bile duct and then removing the suture needle. In the case of using suture needle 5-0, 85-90% of the common bile duct is considered to be obstructed and 75-80% for 4-0. After ligation of the common bile duct, naturally proceeding rats were included as contrast group and rats receiving oral administration of 6 mg/kg/day of folate iron were included as experimental group. After 8 weeks, liver function tests (ALT, AST, ZTT) and histopathological observation of the femur (H-E stained samples) were performed in the contrast and experimental groups, respectively. The significance test for viability and prevalence of osteoporosis following CBD ligation was performed by using the chi-square test, and the significance test for liver function test values was performed by using the t-test. Experimental osteoporosis is induced at 8 weeks after surgical CBD partial ligation (85-90% occlusion). Iron intake significantly suppressed the development of osteoporosis by chronic cholestasis.