The Study on Preparation of Japanese Encephalitis DNA Vaccine
DOI:
https://doi.org/10.46966/msjar.v7i1.347Keywords:
Japanese Encephalitis Virus(JEV), envelope (E) gene, DNA vaccine, pVAX1 vector, recombinant plasmid, ImmunizationAbstract
The major immune antigen gene (JEV E) of JEV was synthesized by RT-PCR and cloned into the eukaryotic expression vector, pVAX1 to prepare recombinant plasmid pVAX-JEV E that could be used as a japanese encephalitis DNA vaccine. In mice (immune groups) vaccinated twice im at 2 weeks intervals with 10 or 50㎍ of JEV DNA vaccine, the antibody titers were 1:160-1:320 and survival rate were 42.8~83.3%. A few mild adverse events were observed in volunteers vaccinated twice with 0.5 mg of JEV DNA vaccine and the antibody titer in vaccines were 1:80 to 1:160 at 2~4 weeks after immunization.
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Copyright (c) 2026 Chang Guk Ri, Yong Su Song, Kyong Hui Hyon, Won Chol Ri, Su Il Pak, Tong Il Choe, Ryong Sik Kim
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