Unmasking the Resistance: Detecting Carbapenem Genes in Acinetobacter baumannii Isolated from some Hospitals in Najaf and Baghdad

Muhammad Kareem  Jasim; Zainab Jaber  Hadi; Hashim Ali Abdualmeer  Alsherees; Aaedah  Annooz

doi:10.46966/msjar.v4i2.127

Unmasking the Resistance: Detecting Carbapenem Genes in Acinetobacter baumannii Isolated from some Hospitals in Najaf and Baghdad

Authors

Muhammad Kareem Jasim Department of Medical Microbiology/ Faculty of Medicine/ University of Kufa.
Zainab Jaber Hadi Department of Medical Microbiology/ Faculty of Medicine/ University of Kufa.
Hashim Ali Abdualmeer Alsherees Department of Medical Microbiology/ Faculty of Medicine/ University of Kufa.
Aaedah Annooz Department of Medical Microbiology/ Faculty of Medicine/ University of Kufa.

DOI:

https://doi.org/10.46966/msjar.v4i2.127

Keywords:

Acinetobacter baumannii, Carbapenem-resistant A. baumannii (CRAB), Carbapenem Genes, Extensive Drug-Resistant (XDR), Modified Hodge test(MHT).

Abstract

Acinetobacter baumannii is a multidrug-resistant bacterium associated with nosocomial infections and known for its ability to develop resistance rapidly. Carbapenem-resistant A. baumannii (CRAB) is a top priority pathogen according to the World Health Organization (WHO). We focused on evaluating the susceptibility of A. baumannii to antibiotics, detecting carbapenemase enzymes using the modified Hodge test, and characterizing the presence of specific carbapenem resistance genes using PCR analysis.This cross-sectional study took place at Al-Sader Medical City and Baghdad Teaching Hospitals from October 2022 to February 2023. It involved 59 A. baumannii isolates collected from patients. The isolates were obtained and processed for accurate diagnosis using morphological techniques, biochemical tests, and Vitek2 systems. The Kirby-Bauer method was employed to assess the susceptibility of the isolates to 24 antibiotics. DNA extraction and PCR analysis were conducted to detect carbapenem resistance genes, 59 specimens from patients, including sputum, wound swabs, blood, and inguinal swabs were analyzed. The majority of isolates were from in-patients, showing a significant difference compared to outpatients. Our finding revealed that among the studied isolates, bla_SPM was the most prevalent gene, detected in 50% of the isolates. This indicates a significant presence of bla_SPM-mediated carbapenem resistance among A. baumannii strains in our study population.Furthermore, our findings demonstrated alarmingly high resistance rates against the majority of antibiotics commonly prescribed to treat A. baumannii infections. A striking 95% of the isolates were classified as extensively drug-resistant, indicating resistance to multiple classes of antibiotics. This poses significant challenges for effective treatment options and underscores the urgent need for alternative strategies in managing A. baumannii infections.

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Published

2023-07-31

How to Cite

Jasim, M. K. ., Hadi, Z. J. ., Alsherees, H. A. A. ., & Annooz, A. . (2023). Unmasking the Resistance: Detecting Carbapenem Genes in Acinetobacter baumannii Isolated from some Hospitals in Najaf and Baghdad. Medical Science Journal for Advance Research, 4(2), 101–111. https://doi.org/10.46966/msjar.v4i2.127

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Issue

Vol. 4 No. 2 (2023): June-July 2023 - Medical Science Journal for Advance Research

Section

Original Articles

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